[e-ISSN : 2277-2782]

Development and Validation of Analytical Method for Simultaneous Estimation of Cephalexin and Probenecid in API and Marketed Formulation By RP-HPLC

International Journal of Novel Trends in Pharmaceutical Sciences,2017,7,6,192-196.
Published:December 2017
Type:Research Article
Author(s) affiliations:

S. Bhagyalaxmi1,*, D. Naresh1, G. Vijaya Kumar1, M.A. Haneef2
1KGR Institute of Technology and Management, Sy. No. 419, Rampally, Keesara, Secunderabad, Telangana- 501301, India.
2Research Scholar, Sri Satya Sai University of Technology and Medical Sciences, Sehore, Madhya Pradesh- 466001, India.


A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Probenecid and Cephalexin, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Zorbax C18 (4.6 x 150mm, 5Jm) column using a mixture of Methanol: Phosphate Buffer pH 3.9 (55:45v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 255nm. The retention time of the Probenecid and Cephalexin was 2.061, 2.462 ±0.02min respectively. The method produce linear responses in the concentration range of 1-5Jg/ml of Probenecid and 100- 500Jg/ml of Cephalexin. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

Calibration graph for Probenecid