Medicinal plants play a key role in the human health care. Phyllanthus amaraus is an economical plant and posess Anti-microbial activity. About 80% of the world Population relies on the use of traditional medicine which is predominately based on plant materials. The traditional medicine refers to a board range of ancient, natural Health care practices including folk/tribal practices as well as Ayurveda, Siddha and Unani. The results of modified agar well diffusion method showed that prepared CWEPA, HWEPA, EEPA, having Inhibitory effect on the microorganisms which are responsible for the intestinal infections, skin infections and urinary tract infection.