The present study was undertaken to isolate and characterize the hydrocarbon degrading microbes from oil contaminated soil samples in Coimbatore, Tamilnadu, India. Totally 113 bacterial isolates were obtained from 60 soil samples. Four were finally selected through secondary screening by an agar well diffusion method using Benzene, Toluene and Xylene (BTX) as carbon source. They were provisionally identified and found closely related to Bacillus cereus, Micrococcus spp, Pseudomonas aeruginosa and E. coli. These isolates selected based on the highest zone of the inhibition on agar well diffusion method. Furthermore, 4 potentials isolate were subjected to gravimetric analysis for determinate the degrading capacity of hydrocarbon degradaters. in this study, Bacillus cereus were degrade the highest amount of BTX, especially Benzene was highly degrade and followed by Toluene. This study clearly demonstrates that Gram-positive bacteria are effective in BTX degradation.