A facile and rapid isocratic reverse phase high performance liquid chromatography assay method has been developed for simultaneous estimation of Ciprofloxacin Hydrochloride and Tinidazole in tablet formulation. The column was equilibrated for at least 30 min and separation was achieved by using inerstil- BDS C18 (250 × 4.6 nm, 5μ). The column was maintained at ambient temperature (27°C). The mobile phase employed was Methanol: Orthophosphoric acid (0.28ml) in 1000 mL of water (55:45 v/v). The eluent was monitored using PDA detector at 245 nm. A volume of 20 μL of standard and sample solutions was injected in to the HPLC. The flow rate was 1.0ml /min. The retention times were 2.955 min and 3.539 for Tinidazole and ciprofloxacin HCl respectively. The developed method was validated as per ICH guidelines. The developed method was found to be accurate, precise and reproducible.