High performance liquid chromatography (HPLC) is an analytical technique which is proficient to separate, detect and quantify various drugs and its related degradants. It is also employed to separate manufactured drugs from drug related impurities, to detect and quantify synthesized drug and to reduce other impurities at the time of separation. A number of chromatographic parameters were evaluated in order to optimize the method. An appropriate mobile phase, column, column temperature, wavelength and gradient must be found that affords suitable compatibility and stability of drug as well as degradants and impurities. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.