Stephania japonica (Thunb). (Syn S. harnendifolia) is a species under the genus of climbers belonging to family Menispermeceae. It is used in traditional medicinal practices and is locally known as Tubuki lota or Goldua. Having high medicinal value, roots of Stephania japonica are used for treatment of fever, diarrhea, dyspepsia and urinary diseases. The alkaloid akanidine shows significant antispasmodic activity on uterine spasms. The leaves of the plant are employed in treatment of convulsions, skin diseases, cough, asthma like symptoms and kidney disorders. Even though this plant has gained scientific importance recently, there is a need for the pharmacognostic standardization. Hence, in the present work the leaf and root part of the plant were subjected to various microscopical and physical evaluations. In the microscopical studies, the different cell structures and arrangements were studied and in physical evaluation the ash values and extractive values were studied. The various pharmacognostical constants were obtained which could help in the development of a suitable monograph for the plant.