[e-ISSN : 2277-2782]

Development of RP - HPLC Method for the Simultaneous determination of Valsartan and Hydrochlorothiazide in Bulk and in Pharmaceutical Formulation

International Journal of Novel Trends in Pharmaceutical Sciences,2011,1,1,18-22.
Published:October 2011
Type:Research Article
Authors:
Author(s) affiliations:

Jothieswari. D1*, Priya. D2, Brito Raj. S3, Mohanambal. E3, Wasim Raja. S4

1Department of Pharmaceutical Analysis, Sri Venkateswara College of Pharmacy, Chittoor, Andhra Pradesh, India.

2Department of Pharmaceutical Chemistry, Sri Venkateswara College of Pharmacy, Chittoor, Andhra Pradesh, India.

3Department of Pharmaceutics, Sri Venkateswara College of Pharmacy, Chittoor, Andhra Pradesh, India.

4Department of Pharmacy Practice, Sri Venkateswara College of Pharmacy, Chittoor, Andhra Pradesh, India.

Abstract:

A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of valsartan and hydrochlorothiazide in bulk and in pharmaceutical formulation using RP - C18 column. The mobile phase (acetonitrile: methanol: 50 mM phosphate buffer adjusted to pH 3 with orthophosphoric acid) was pumped at a flow rate of 1.0 ml/ min in the ratio of 20: 50: 30%v/v and the eluents were monitored at 250 nm. Linearity was obtained in the concentration range of 4 - 40 mg/ ml for valsartan and 1 – 10 mg/ ml for hydrochlorothiazide. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining valsartan and hydrochlorothiazide in bulk and in pharmaceutical dosage form.

Optimized chromatogram for HCT and VAL (10 mg